238000001042 affinity chromatography Methods 0.000 title claims abstract description 19.238000011012 sanitization Methods 0.000 title claims abstract description 36.
Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority claimed from GB1503578.5 external-priority Application filed by Ge Healthcare Bioprocess R&D Ab filed Critical Ge Healthcare Bioprocess R&D Ab Priority to CN201680013453.5A priority Critical patent/CN107405541A/en Priority to JP2017545957A priority patent/JP6732292B2/en Priority to US15/554,075 priority patent/US20180036445A1/en Priority to EP16706227.2A priority patent/EP3265198A1/en Publication of WO2016139128A1 publication Critical patent/WO2016139128A1/en Links Inventor Elin Marianne MONIE Tomas Bjorkman Anna Gronberg Anders Ljunglof Gustav Jose RODRIGO Karin TORSTENSON Magnus Carl Erik WETTERHALL Original Assignee Ge Healthcare Bioprocess R&D Ab Priority date (The priority date is an assumption and is not a legal conclusion. Google Patents WO2016139128A1 - Sanitization method for affinity chromatography matrices Thus, it is important to optimize the purification protocol to achieve efficient capture and maximum recovery of the target.WO2016139128A1 - Sanitization method for affinity chromatography matrices Affinity purification can provide significant time savings and several hundred-fold or higher purification, but the success depends on the method used. In recent years, matrices with unique features which overcome some of the limitations of more traditional materials have been developed and these are also described.
In this chapter, we discuss factors which are important to consider when selecting the ligand, proper attachment chemistry, and the matrix.
However, in some cases new affinity chromatographic material may need to be developed by coupling the ligand onto the matrix such that the ligand retains specific binding affinity for the molecule of interest. With the growing popularity of affinity purification, many of the commonly used ligands coupled to affinity matrices are now commercially available and are ready to use. Successful affinity purification requires a certain degree of knowledge and understanding of the nature of interactions between the target molecule and the ligand to help determine the selection of an appropriate affinity ligand and purification procedure. These interactions, which are typically reversible, are used for purification by placing one of the interacting molecules, referred to as affinity ligand, onto a solid matrix to create a stationary phase while the target molecule is in the mobile phase. It is based on highly specific biological interactions between two molecules, such as interactions between enzyme and substrate, receptor and ligand, or antibody and antigen. Affinity chromatography is one of the most diverse and powerful chromatographic methods for purification of a specific molecule or a group of molecules from complex mixtures.
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